mouse heart tissue  (Bertin Technologies)

Journal: Acta Materia Medica

Article Title: Cryptotanshinone alleviates vasculitis in Kawasaki disease by modulating macrophage-neutrophil interactions

doi: 10.15212/amm-2024-0076

Figure Lengend Snippet: Figure 1 | CTS alleviates disease severity in the LCWE mouse KD model. A. Schematic diagram of KD model construction in LCWE-induced KD mice. B. Body temperatures of mice in the normal, LCWE, high-dose CTS, and low-dose CTS groups during 2 weeks of model construction. C. Representative images of the abdominal area in the four groups of mice, and statistical analysis of abdominal aortic diameter. Scale bars, 200 mm. D. Representative H&E images of heart tissue, coronary arteries, and aortic root regions in the four groups of mice. Scale bars, 50 μm.

Article Snippet: For immunohistochemistry, mouse heart tissue paraffin sections were deparaffinized, rehydrated, subjected to antibody retrieval with sodium citrate, blocked, stained with anti-F4/80 antibody (Cell Signaling Technology, cat. no. 30325T) at a dilution of 1:100, and incubated overnight at 4°C.

Techniques:

Journal: Acta Materia Medica

Article Title: Cryptotanshinone alleviates vasculitis in Kawasaki disease by modulating macrophage-neutrophil interactions

doi: 10.15212/amm-2024-0076

Figure Lengend Snippet: Figure 5 | CTS treatment decreases macrophage-neutrophil interactions in mouse heart tissue.

Article Snippet: For immunohistochemistry, mouse heart tissue paraffin sections were deparaffinized, rehydrated, subjected to antibody retrieval with sodium citrate, blocked, stained with anti-F4/80 antibody (Cell Signaling Technology, cat. no. 30325T) at a dilution of 1:100, and incubated overnight at 4°C.

Techniques: